Abstract
P 091
Gene expression analysis of goosecoid homebox 2, B-cell leukemia/ lymphoma 6, oligophrenin 1 and Ly6 / neurotoxin 1 in mouse retina during embryogenesis
Maja Kaiser1, D. Kaiser1, H. Nakhai1, L. Mendoza1, Thomas Kalinski2, Gernot I. W. Duncker1, Lars Bräuer3, Friedrich Paulsen3, Saadettin Sel1
1Universitäts-Augenklinik, Martin-Luther-Universität Halle-Wittenberg, Halle/Saale, 2Institut für Pathologie, Universität Magdeburg, 3Institut für Anatomie und Zellbiologie, Martin-Luther-Universität Halle-Wittenberg, Halle/Saale
Objective
Precise localization of expressed genes in the retina (rods, coins, ganglial-, amacrine-, horizontal-, bipolar– and Mueller cells). The expression analysis included a time dependent aspect with respect to animal`s age (E 12, 14, 16, 18, P 0, 1, 4, 7, 14, 30, 175).
Methods
A strategy is applied that is based on PCR and multiple antigen immunostaining of the protein of interest simultaneously to cell specific retinal marker (for differentiation between rods, coins, ganglial -, amacrine -, horizontal -, bipolar – and Mueller cells).
Results
Our analysis revealed the expression of the genes goosecoid homebox 2 (Gsc2), B-cell leukemia/lymphoma 6 (Bcl6), oligophrenin 1 (ophn1) and Ly6/neurotoxin 1 (lynx1) during the retinogenesis.
Conclusions
This study provides basic information for our understanding of gene function during retinogenesis.
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