Abstract

P 146

The role of y+-transporter in pathogenese of ocular surface diseases

Kristin Jäger, Lars Bräuer, Fabian Garreis, Friedrich Paulsen
Institut für Anatomie und Zellbiologie, Halle/Saale

Many diseases which result from l-arginine deficiency (diabetes, chronic renal failure, psoriasis) are assoziate with dry eye syndrom and oculare surface inflammation. In investigations of dry eye one very important fact was ingnored always, the y+-transporter. The transporter covered 80 percent of l-arginin-transport in human. He transports the cationic amino acids l-arginine, l-ornithine and l-lysine exclusive and will represented by cationic amino acid transporter proteins (CAT-proteins). Own preliminaries located the human CATs in ocular surface for the first time.
Afterwards the y+-transporter should be associated with inflammations of ocular surface and dry eye syndrome. For this cornea epithelial cells were stimulated with heat inactivated supernatant of Pseudomonas aeruginosa and Staphylococcus aureus and Th1-cytokines (TNFa, IL-1b and INFg). Additionally the cells were irriatiated with UVB. The interpretation of this investigation was made by realtime-PCR. Furthermore was made immunohistochemical stain with hCAT1 and hCAT2 antibodies of pathological (Fuchs dystrophy, Herpes keratitis, bullouse Keratopathy, Keratoconus, corneal Ulceration with S. aureus infection) and healthy corneas. The interpretation of the stain showed a different hCAT1 and hCAT2- proteinexpression of pathological corneas in comparsion with healthy corneas. The stimulation of heat inactivated supernatant of S. aureus or TNFa showed a significant increase of hCAT1- and 2 mRNA- expression, hCAT2 was stimulate of heat inactivated supernatant of P. aeruginosa also. HCAT1 was significant decreased by UVB-irradiation. This result show that y+-transporter is different regulate under pathological conditions. So he is an appropriate candidate for therapeutical interventions for diseases of ocular surface.

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