Abstract

P 203

Cross-linked hyaluronic acid as vitreous replacment – in-vivo biocompatibility

Martin S. Spitzer, Charlotte Frank, Max Warga, Maximilian Schultheiss, Karl U. Bartz-Schmidt, Peter Szurman
Department für Augenheilkunde, Eberhard-Karls-Universität Tübingen, Tübingen

Aim
To test the biocompatibility and long-term stability of cross-linked hyaluronic acid biopolymers as vitreous replacement in rabbit eyes.
Methods
Hyaluronic acid was cross-linked using a modified protocol. The vitreous was removed by pars plana vitrectomy in 10 rabbit eyes and replaced by injection of the cross-linked biogels. ERG, slit-lamp examination, funduscopy and measurement of intraocular pressure were performed at regular intervals postoperatively. The eyes were enucleated 4 to 10 weeks after surgery and histology was assessed for signs of cytotoxicity.
Results
The Biogels could be injected without complications via the pars plans using Weber cannulas. There were no signs of intraocular inflammation and intraocular pressure was normal during the entire postoperative period. Electrophysiology, histology and immunohistochemistry demonstrated an excellent tissue biocompatibility. The biogels remained in place for up to 10 weeks without losing optical clarity or elasticity.
Conclusion
The results demonstrate the general feasibility and biocompatibility of cross-linked hyaluronic acid biopolymers as vitreous replacement.

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