Abstract

DO.19.07

The safety profile of alkylphosphocholine in the model of the isolated perfused vertebrate retina

Matthias Lüke1, Kai Januschowski2, Julia Lüke1, Klaus Dietz3, Anselm Kampik4, Karl-Ulrich Bartz-Schmidt2, Kirsten Eibl5
1Klinik für Augenheilkunde, Campus Lübeck, Universitätsklinikum Schleswig-Holstein, Lübeck, 2Universitäts-Augenklinik Tübingen, 3Abteilung für Medizinische Biometrie, Eberhard-Karls-Universität Tübingen, Tübingen, 4Augenklinik der Ludwig-Maximilians-Universität München, München, 5Campus Innenstadt, Augenklinik der LMU München, München

Objective
(APC) are synthetic phospholipid derivatives and have been demonstrated to inhibit ocular cell proliferation in vitro and in vivo. Currently, they are applied clinically for their antitumoral and antiparasitic properties, but have not yet been implemented for clinical use in proliferative ophthalmic disorders. The purpose of this study was to assess the safety of APC in the ex vivo model of the perfused vertebrate retina.
Methods
Bovine retina preparations were perfused with oxygen pre-equilibrated standard solution. The electroretinogram (ERG) was recorded using Ag/AgCl-electrodes. After recording stable b-wave amplitudes, APC was applied at the following concentrations to the nutrient solution: 0.25µM, 2.5µM and 25µM. To disclose the effects of alkylphosphocholine on photoreceptor function a test series at the same concentrations were performed to evaluate the effects of APC on the a-wave amplitude. Aspartate at a concentration of 1mM was added to the nutrient solution to obtain stable a-wave amplitudes. Thereafter, APC was applied at the same concentrations to the nutrient solution. The recovery of the ERG-amplitudes was followed up for 75 minutes.
Results
No reductions of the a- and b-wave amplitude were found at the end of the exposure with APC in each test series. No differences were found between the ERG-amplitudes before and after application of APC at the end of the washout.
Conclusions
In this ex vivo model of the perfused vertebrate retina, APCs have proved to be a safe compound in the concentrations applied. Thus, APCs should further be considered as promising candidates for future clinical applications in ophthalmology.

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